
According to "Jungle" lore, anything is funny as long as "Yakety Sax" is playing at the same time. Jim Rome plays "Yakety Sax" numerous times during a program, making it a big running joke on the show. When a sports figure makes a bad move or is wronged, he will usually play it preceding a line, trying to illustrate that "Yakety Sax" makes everything better. Jim Rome, host of the nationally syndicated sports talk show The Jim Rome Show, uses "Yakety Sax" to make light of bad situations. The piece was also featured in The Simpsons' episode " A Star Is Born - Again".
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A variation of the theme was used in an episode of animated TV series Back at the Barnyard and in the episode Quest for Ratings of the cartoon series South Park.
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It also appears in the 2006 Dreamworks movie Flushed Away.

The piece is also used in the 1987 comedy film Rags to Riches with Joseph Bologna and Douglas Seale.

The piece is featured in the 2005 film V for Vendetta, playing during a TV comedy skit which Gordon Dietrich, played by Stephen Fry, writes without censor approval. This use of the piece, and the chase scenes themselves, have been parodied in many other movies and TV shows. It is frequently used to accompany comedic chases, most notably in the sketch comedy program The Benny Hill Show, where it accompanied otherwise silent, rapidly paced comedy sequences (often involving a chase scene). "Yakety Sax" is often used in television and film as a soundtrack for outlandishly humorous situations.

Two bars of " Entrance of the Gladiators" are also worked into it. The composition includes pieces of assorted fiddle tunes such as " Chicken Reel", and was written for a performance at a venue called The Armory in Hopkinsville, Kentucky. "Spider" Rich and popularized by saxophonist Boots Randolph. "Yakety Sax" is a piece of music written by James Q. Music and photo's of Boots Randolph and Benny Hill Yakety Sax (Theme from Benny Hill Show) / Boots Randolph
